Biosurfactant production from marine hydrocarbon-degrading consortia and pure bacterial strains using crude oil as carbon source.

摘要: Biosurfactants (BS) are “green” amphiphilic molecules produced by microorganisms during biodegradation, increasing the bioavailability of organic pollutants. In this work, BS production yield marine hydrocarbon degraders isolated from Elefsina bay in Eastern Mediterranean Sea has been investigated. The drop collapse test was used as a preliminary screening to confirm biosurfactant producing strains or mixed consortia. community structure best consortia based on determined 16S-rDNA pyrotag screening. Subsequently, effect incubation time, temperature, substrate and supplementation with inorganic nutrients, production, examined. Two types - lipid mixtures were extracted culture broth; low molecular weight Rhamnolipids Sophorolipids. Crude extracts purified silica gel column chromatography then identified thin layer (TLC) Fourier transform infrared spectroscopy (FT-IR). Results indicate that remains constant while it is independent total biomass, carbon source, temperature. A concentration broth continuous degradation crude oil implies microbes generate no more than required amount biosurfactants enables biodegradation oil. Isolated pure found have higher specific yields complex microbial community-consortia. heavy fraction emerged promising for (by producers) fewer impurities final product. Furthermore, particular strain sediments, Paracoccus marcusii, may be an optimal choice bioremediation purposes its biomass trapped phase, not suffering potential dilution effects sea currents.