Hansenula polymorpha (Pichia angusta)

摘要: A small and closely related group of yeasts is capable using methanol as sole source carbon energy. Among these methylotrophic organisms, Hansenula polymorpha in particular has gained increasing attention recent years, both basic applied sciences. This mainly due to two physiological features that are an integral part the methanol-utilizing machinery this yeast. One expression huge amounts key methanol-metabolizing enzymes, which can, case oxidase, amount up one third total cellular protein. The driven by very strong promoters form basis for a highly competitive system produce foreign proteins at industrial scale (Gellissen et al. 1994). other prominent characteristic H. accompanied dramatic growth proliferation microbodies, i.e., peroxisomes (Veenhuis Harder 1987). thus served and, addition introduction systems such Saccharomyces cerevisiae, still continues serve valuable model organism study biogenesis organelles. Although we will briefly discuss contributions field peroxisome research, our main focus within chapter belongs practical aspects importance working with