Aurora A Kinase Contributes to a Pole-Based Error Correction Pathway

摘要: Chromosome biorientation, where sister kinetochores attach to microtubules (MTs) from opposing spindle poles, is the configuration that best ensures equal partitioning of genome during cell division. Erroneous kinetochore-MT attachments are commonplace but often corrected prior anaphase. Error correction, thought be mediated primarily by centromere-enriched Aurora B kinase (ABK), typically occurs near poles; however, relevance this locale unclear. Furthermore, polar ejection forces (PEFs), highest can stabilize improper pushing mal-oriented chromosome arms away poles. Hence, there a conundrum: erroneous weakened PEFs most likely strengthen them. Here, we report A (AAK) opposes stabilizing effect PEFs. AAK activity contributes phosphorylation kinetochore substrates poles and its inhibition results in misalignment an increased incidence attachments. directly phosphorylates site N-terminal tail Ndc80/Hec1 has been implicated reducing affinity Ndc80 complex for MTs when phosphorylated. We propose gradient correcting vicinity