Toxins from the entomogenous fungus Metarrhizium anisopliae. I. Production in submerged and surface cultures, and in inorganic and organic nitrogen media.

摘要: Abstract An isolate of Metarrhizium anisopliae from the eastern United States was found to produce substances which caused tetanic paralysis in larvae greater wax moth, Galleria mellonella , when injected into hemocoel. The production these materials vitro investigated and it that they were present amounts submerged cultures than surface cultures, even dry mycelial weights approximately equal. toxins produced with either inorganic or organic nitrogen as source culture medium. They released by growing colonies titers did not increase lysed, indicates exotoxins. A bioassay system devised for determining nonlethal toxins. criterion positive response inability test larva ( ) right itself placed on its back 5 minutes after injection. ED 50 's estimated plotting normit against logarithm dose (in microliters per gram larval weight).