Identification of the Heme Compound Copurified with Deoxyribonucleic Acid (DNA) from Bloodstains, a Major Inhibitor of Polymerase Chain Reaction (PCR) Amplification

摘要: The heme compound found in deoxyribonucleic acid (DNA) extracted from bloodstains, which is regarded as a major inhibitor of polymerase chain reaction (PCR), was characterized comparison with alkaline and hematin, histidine ammonia hemochromogens, globin serum albumin hemochromogens digested by proteinase K. Alkaline hematin were almost completely removed phenol/chloroform treatment ethanol precipitation, so not to be copurified DNA the specimens. Spectrophotometric results indicated that contaminant likely product K digestion some heme-blood protein complex, organic solvents remained precipitates DNA. polyacrylamide gradient gel electrophoresis intensity inhibition PCR suggested ligand somewhat large molecule, resistant proteolysis addition bovine mixture prevented compounds, probably binding heme. This showed due irreversible inactivation enzyme.