Reflections of a lucid dreamer: optical trap design considerations.

摘要: Publisher Summary This chapter discusses the optical trap design considerations. The technique can be used to constrain and move small particles in solution using a light microscope laser beam. Trapping size scales sensitivity are well suited for studying mechanical properties of single cells, organelles, even molecules. describes considerations involved planning implementation an trapping high-resolution force displacement measurements trapped particles. These molecules will bind actin filament, allowing measurement their at molecule level. illustrates observations that these beads with nanometer resolution use active feedback loops suppress bead diffusion by rapid deflection, observethe specimen brightfield fluorescent imaging simultaneously.