The push-pull mechanism of bacteriophage Ø29 DNA injection
作者： Víctor González-Huici , Margarita Salas , José M. Hermoso
DOI: 10.1111/J.1365-2958.2004.03993.X
关键词:
摘要: The mechanism of bacteriophage DNA injection is poorly understood, often considered a simple process, driven merely by the packing pressure inside capsid. In contrast to well-established packaging Bacillus subtilis phage O29, that involves molecular motor formed connector and viral ATPase, nothing known about its into cell. We have studied this process measuring binding p6, genome organization protein. linear penetrates with right-left polarity, in two-step process. first step approximately 65% pushed cell most probably built Thus, synthesis proteins from right early operon allowed. This controlled, bacterial protein(s) slow down entry. second at least one proteins, p17, participates machinery pulls remaining Both steps are energy-dependent, as treatment cells azide overrides whole mechanism, leading deregulated, passive entry DNA.
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Abdelkarim Filali Maltouf, Bernard Labedan, The energetics of the injection process of bacteriophage lambda DNA and the role of the ptsMpel-encoded protein Biochemical and Biophysical Research Communications. ,vol. 130, pp. 1093- 1101 ,(1985) , 10.1016/0006-291X(85)91728-0
Paola Crucitti, José M Lázaro, Vladimı́r Beneš, Margarita Salas, BACTERIOPHAGE O29 EARLY PROTEIN P17 IS CONDITIONALLY REQUIRED FOR THE FIRST ROUNDS OF VIRAL DNA REPLICATION Gene. ,vol. 223, pp. 135- 142 ,(1998) , 10.1016/S0378-1119(98)00167-X
P Boulanger, L Letellier, Ion channels are likely to be involved in the two steps of phage T5 DNA penetration into Escherichia coli cells. Journal of Biological Chemistry. ,vol. 267, pp. 3168- 3172 ,(1992) , 10.1016/S0021-9258(19)50710-4
Ian J. Molineux, No syringes please, ejection of phage T7 DNA from the virion is enzyme driven. Molecular Microbiology. ,vol. 40, pp. 1- 8 ,(2001) , 10.1046/J.1365-2958.2001.02357.X
Shelley Grimes, Paul J. Jardine, Dwight Anderson, Bacteriophage φ29 DNA packaging Advances in Virus Research. ,vol. 58, pp. 255- 294 ,(2002) , 10.1016/S0065-3527(02)58007-6
Ana CAMACHO, Fernando JIMENEZ, Javier TORRE, Jose L. CARRASCOSA, Rafael P. MELLADO, Eladio VINUELA, Margarita SALAS, Cesar VASQUEZ, Assembly of Bacillus subtilis phage phi29. 1. Mutants in the cistrons coding for the structural proteins. FEBS Journal. ,vol. 73, pp. 39- 55 ,(1977) , 10.1111/J.1432-1033.1977.TB11290.X
A Filali Maltouf, B Labedan, Host cell metabolic energy is not required for injection of bacteriophage T5 DNA. Journal of Bacteriology. ,vol. 153, pp. 124- 133 ,(1983) , 10.1128/JB.153.1.124-133.1983
Douglas E. Smith, Sander J. Tans, Steven B. Smith, Shelley Grimes, Dwight L. Anderson, Carlos Bustamante, The bacteriophage φ29 portal motor can package DNA against a large internal force Nature. ,vol. 413, pp. 748- 752 ,(2001) , 10.1038/35099581
P Boulanger, L Letellier, Characterization of ion channels involved in the penetration of phage T4 DNA into Escherichia coli cells. Journal of Biological Chemistry. ,vol. 263, pp. 9767- 9775 ,(1988) , 10.1016/S0021-9258(19)81584-3
Peixuan Guo, Structure and function of φ29 hexameric RNA that drives the viral DNA packaging motor: Review Progress in Nucleic Acid Research and Molecular Biology. ,vol. 72, pp. 415- 472 ,(2002) , 10.1016/S0079-6603(02)72076-X