Analysis of Carotenoids Using LC-MS-MS with Ion Mobility Spectrometry and Photoionization

摘要: Carotenoids are naturally occurring fat-soluble pigments found principally in bacteria and plants including edible plants. Because of their extensively conjugated carbon-carbon double bond system, carotenoids potent antioxidants. The most abundant carotenoid best singlet oxygen quencher red tomatoes is lycopene. Using lycopene as a potential dietary chemopreventive agent, four-arm randomized, placebo-controlled, double-blind phase II clinical intervention trial was carried out at the University Illinois Chicago. In investigation, it that supplements either 30 mg/d or 60 for 21 d were well tolerated by healthy men enrolled to two arms. Similar increases serum levels observed both treatment groups during this study. However, stronger effects antioxidant biomarkers (urinary 8-oxo-deoxyguanosine 8-iso-prostaglandin-F2) with high dose arm compared its placebo control. Even though significant progress has been made analyzing carotenoids, there continuing demand more efficient sensitive analytical methods. Technologies have seldom applied analysis ion mobility spectrometry photoionization mass explored utility area. The use LC-IM-tandem helped confirm in-source isomerization lycopene, β-carotene, lutein, zeaxanthin occurred positive electrospray. This be temperature dependent. After separation using IM, cis all-trans isomers shown MS-MS CID produce unique fragmentation patterns could used distinguish them. Atmospheric pressure spectrometric ionization technique new analysis. study, we first reported can ionized negative APPI. It also suggested dopants essential formation ions Among evaluated, methyl-tert-butyl ether particularly effective towards As APPI, anisole chlorobenzene effective.