Downregulation of SEMA4C Inhibit Epithelial-Mesenchymal Transition (EMT) and the Invasion and Metastasis of Cervical Cancer Cells via Inhibiting Transforming Growth Factor-beta 1 (TGF-β1)-Induced Hela cells p38 Mitogen-Activated Protein Kinase (MAPK) Activation
作者： Lilan Yang , Yayuan Yu , Zhenfang Xiong , Hongxia Chen , Buzhen Tan
DOI: 10.12659/MSM.918123
关键词:
摘要: BACKGROUND Epithelial-mesenchymal transition (EMT) plays a key role in promoting invasion and metastasis of tumor cells. SEMA4C can regulate the generation transforming growth factor-beta 1 (TGF-s1)-induced EMT cervical cancer. This study investigated relationship between regulation on TGF-s1-induced p38 mitogen-activated protein kinase (MAPK) activation MATERIAL AND METHODS Hela-shSEMA4C cell line was established success transfection confirmed with fluorescence intensity. Cell experiments were divided into 2 groups. Group Hela, Hela-shNC, Hela-shSEMA4C; Hela-shSEMA4C, Hela+TGF-s1, Hela-shNC+TGF-s1, Hela-shSEMA4C+TGF-s1. detected for mRNA expression by real-time polymerase chain reaction (RT-PCR), viability Counting Kit-8 (CCK-8), F-actin intensity immunofluorescence, migration scratch test, test. analyzed E-cadherin human fibronectin (FN) content enzyme-linked immunosorbent assay (ELISA), SEMA4C, p-p38 expressions Western blot. RESULTS For 1, compared Hela Hela-shNC subgroups, expression, viability, intensity, ability subgroup significantly decreased (P<0.05). 2, increased (P<0.01), while FN content, MAPK (P<0.01). Compared Hela-shNC+TGF-s1 Hela+TGF-s1 Hela-shSEMA4C+TGF-s1 CONCLUSIONS Downregulation inhibit cancer cells via inhibiting activation.
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