lncRNA H19 regulates epithelial-mesenchymal transition and metastasis of bladder cancer by miR-29b-3p as competing endogenous RNA.

摘要: Accumulating evidences indicate that long noncoding RNAs (lncRNAs) might play important roles in tumorigenesis and metastasis. EMT (epithelial-to-mesenchymal transition) is considered as a critical step invasion metastasis of various tumors including bladder cancer (BC). Recent researches have showed lncRNA H19 implicated through regulating the reverse MET (mesenchymal-to-epithelial transition). However, underlying mechanisms remain largely unknown. Here, we screened mRNA expression profiles BC with microarray assay. We found DNMT3B displayed higher co-expression tissues cells. Functionally, demonstrated could increase proliferation, migration, regulate well rearrange cytoskeleton cells vitro. Moreover, ectopic promoted tumorigenesis, angiogenesis pulmonary vivo, whereas knockdown has contrary role vivo Mechanistically, proved directly bind to miR-29b-3p (miR-29b) derepress target DNMT3B. co-localization. More importantly, up-regulating antagonized miR-29b-3p-mediated migration suppression Furthermore, partially reversed function inhibitor on facilitated miR-29b-3p-induced MET. Taken together, for first time ceRNA (competing endogenous RNA) relieve DNMT3B, which led BC. Our findings highlight novel mechanism progression provide H19/miR-29b-3p/DNMT3B axis promising therapeutic