Assessment of the invasive potential of human gynecological tumor cell lines with the in vitro Boyden chamber assay: influences of the ability of cells to migrate through the filter membrane

摘要: The Boyden chamber assay is widely used for in vitro measurement of the invasive capacity cells. However, results can be affected significantly if certain precautions are not taken. Using we investigated potential a variety human gynecological tumor cell lines to degrade and migrate through artificial basement membrane matrix Matrigel. absence this Matrigel layer large differences were observed ability cells adhere to, attach lower side filter membranes. These influenced by density, degree directional locomotion, size pores. To adjust these influences (which directly correlated traverse layer), invasion corrected membrane. In addition, MDA-MB-231 was as an internal standard compensate variations between different experiments. Overall, our experimental set up, five breast cancer most (mean ± SEM relative invasion: 104.7 6.1%), ovarian least (60.2 2.2%) six endometrial showed intermediate (79.1 3.5%). conclusion, reliably studying vitro, taken into account, reference line included enable comparison data obtained from independently performed experiments on lines.