Transcriptome analysis and functional validation reveal a novel gene, BcCGF1, that enhances fungal virulence by promoting infection‐related development and host penetration

摘要: Simultaneous transcriptome analyses of both host plants and pathogens, functional validation the identified differentially expressed genes (DEGs) allow us to better understand mechanisms underlying their interactions. Here, we analyse mixed derived from Botrytis cinerea (the causal agent grey mould) infected tomato leaves at 24 hr after inoculation, a critical time point which pathogen has penetrated developed in leaf epidermis, whereas necrotic symptoms have not yet appeared. Our complex network involved tomato-B. interaction. The expression fungal transcripts encoding candidate effectors, enzymes for secondary metabolite biosynthesis, hormone reactive oxygen species (ROS) production, autophagy-related proteins was up-regulated, suggesting that these may be initial infection processes. Specifically, phytoalexin stress responses, ATP-binding cassette transporters, pathogenesis-related proteins, WRKY DNA-binding transcription factors were up-regulated. We functionally investigated several B. DEGs via gene replacement pathogenicity assays, demonstrated BcCGF1 novel virulence-associated factor mediates development virulence regulation conidial germination, conidiation, structure formation, penetration, adaptation. infection-related controlled by BcCGF-mediated ROS production exogenous cAMP restored mutant development. findings provide new insights into elucidation simultaneous tactics attack defence. systematic mediating pathogenesis open up targets disease control.