Mycobacterium tuberculosis Phosphate Uptake System Component PstA2 Is Not Required for Gene Regulation or Virulence

摘要: The Mycobacterium tuberculosis genome encodes two complete high-affinity Pst phosphate-specific transporters. We previously demonstrated that a membrane-spanning component of one system, PstA1, was essential both for M. virulence and regulation gene expression in response to external phosphate availability. To determine if the alternative system is similarly required or regulation, we constructed deletion pstA2. Transcriptome analysis revealed PstA2 not phosphate-replete growth conditions. also dispensable replication mouse aerosol infection model. However, ΔpstA1ΔpstA2 double mutant attenuated mice lacking cytokine interferon-gamma, suggesting requires transport survive limitation encountered host. Surprisingly, ΔpstA2 bacteria were more resistant acid stress vitro. This phenotype intrinsic transporter since ΔpstS1 exhibited similar resistance. Our data indicate transporters have distinct physiological functions, with PstA1 being specifically involved sensing while influences survival acidic