Synthesis of oligo- and polysaccharides by Lactobacillus reuteri 121 reuteransucrase at high concentrations of sucrose.

摘要: GTFA, a glucansucrase enzyme of the probiotic bacterium Lactobacillus reuteri 121, is capable synthesizing an α-glucan polysaccharide with (1 → 4) and 6) linkages from sucrose. With respect to its biosynthesis, present study has shown that ratio oligosaccharide versus synthesized was directly proportional concentration It appears size distribution products kinetically controlled, but linkage in material not changed. At high sucrose concentrations isomers leucrose trehalulose were synthesized, using accumulated fructose as acceptor, together 4'- 6'-α-D-glucosyl-leucrose 6'-α-D-glucosyl-trehalulose. The finding additional branched hexasaccharide demonstrates able introduce branch-points already relatively short oligosaccharides.