Transcriptome Changes in Colorectal Cancer Cells upon Treatment with Avicequinone B

摘要: Purpose: Naphtho[2,3-b]furan-4,9-dione (Avicequinone B), a natural naphthoquinone isolated from the mangrove tree Avicennia alba , is recognized as valuable synthetic precursor with anti-proliferative effect. However, molecular mechanism involved in its bioactivity has not been investigated. This study aimed to determine selectivity of avicequinone B against cancer cells and transcriptomic changes induced colorectal (CRC). Methods: The cytotoxic effect adenocarcinoma-derived or fibroblasts was evaluated using MTT assay. In addition, CRC were treated different settings evaluate colony-forming ability, cell cycle progression, apoptosis/necrosis induction, transcriptome response by RNA-seq. Results: Avicequinone effectively reduced viability breast, colorectal, lung adenocarcinoma IC50 lower than 10 μM, while less affected. induction G2/M arrest necrosis-like death observed B-treated HT-29 cells. Furthermore, RNA-seq revealed 490 differentially expressed genes, highlighting reduction interferon stimulated genes proliferative signaling pathways (JAK-STAT, MAPK, PI3K-AKT), well ferroptosis miR-21 expression. Conclusion: short, these results demonstrated therapeutic potential paved foundation for elucidating mechanisms context CRC.