Mutation breeding of Saccharomyces cerevisiae with lower methanol content and the effects of pectinase, cellulase and glycine in sugar cane spirits.
作者： Ming-Hua Liang , Ying-Jie Liang , Xiao-Na Wu , Shi-Shui Zhou , Jian-Guo Jiang
DOI: 10.1002/JSFA.6908
关键词:
摘要: BACKGROUND To decrease the methanol content of sugar cane sprits, mutagenesis ultraviolet (UV) coupled with diethyl sulfate (DES) was used to generate a mutant Saccharomyces cerevisiae lower content. Meanwhile, effects additions pectinase, cellulase and glycine on production in spirits were evaluated. RESULTS After UV DES, S. DU9 low (97.3 ± 1.7 mg/L) selected, 12.3% that D4 only DES treatment, 27.8% reduction strain without any treatment. Pectinase significantly increased levels spirits. The results showed there linear relationship between (concentration within 0∼0.9 g/L) sprits equation y = 104.7 × −4.79 conversion rate as 24.56%. CONCLUSION Mutagenesis is an efficient way Also, it necessary control fermentation medium, other unknown ways metabolic pathway yeasts may need further study. © 2014 Society Chemical Industry
sci-hub.se 参考文章(25)
WO Okunowo, AA Osuntoki, RO Okotore, The alcoholic fermentative efficiency of indigenous yeast strains of different origin on orange juice African Journal of Biotechnology. ,vol. 4, pp. 1290- 1296 ,(2005) , 10.4314/AJB.V4I11.71403
Sergio Gómez-Alonso, Isidro Hermosín-Gutiérrez, Esteban García-Romero, Simultaneous HPLC analysis of biogenic amines, amino acids, and ammonium ion as aminoenone derivatives in wine and beer samples. Journal of Agricultural and Food Chemistry. ,vol. 55, pp. 608- 613 ,(2007) , 10.1021/JF062820M
M.K. Bhat, Cellulases and related enzymes in biotechnology Biotechnology Advances. ,vol. 18, pp. 355- 383 ,(2000) , 10.1016/S0734-9750(00)00041-0
Dulce M Díaz-Montaño, Ernesto Favela-Torres, Jesus Córdova, Improvement of growth, fermentative efficiency and ethanol tolerance of Kloeckera africana during the fermentation of Agave tequilana juice by addition of yeast extract Journal of the Science of Food and Agriculture. ,vol. 90, pp. 321- 328 ,(2010) , 10.1002/JSFA.3820
Purificación Hernández-Orte, Juan F. Cacho, Vicente Ferreira, Relationship between Varietal Amino Acid Profile of Grapes and Wine Aromatic Composition. Experiments with Model Solutions and Chemometric Study Journal of Agricultural and Food Chemistry. ,vol. 50, pp. 2891- 2899 ,(2002) , 10.1021/JF011395O
Juan Octavio Valle-Rodríguez, Guillermo Hernández-Cortés, Jesús Córdova, Mirna Estarrón-Espinosa, Dulce María Díaz-Montaño, Fermentation of Agave tequilana juice by Kloeckera africana: influence of amino-acid supplementations Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology. ,vol. 101, pp. 195- 204 ,(2012) , 10.1007/S10482-011-9622-X
Maurizio Simmaco, Daniela De Biase, Donatella Barra, Francesco Bossa, None, Automated amino acid analysis using precolumn derivatization with dansylchloride reversed-phase high-performance liquid chromatography Journal of Chromatography A. ,vol. 504, pp. 129- 138 ,(1990) , 10.1016/S0021-9673(01)89519-4
Sarah Croak, Milena Corredig, The role of pectin in orange juice stabilization: Effect of pectin methylesterase and pectinase activity on the size of cloud particles Food Hydrocolloids. ,vol. 20, pp. 961- 965 ,(2006) , 10.1016/J.FOODHYD.2005.10.016
Isabel Revilla, María Luisa González-SanJosé, Methanol release during fermentation of red grapes treated with pectolytic enzymes Food Chemistry. ,vol. 63, pp. 307- 312 ,(1998) , 10.1016/S0308-8146(98)00049-1
Xian Zhang, Rongzhen Zhang, Taowei Yang, Jing Zhang, Meijuan Xu, Huazhong Li, Zhenghong Xu, Zhiming Rao, Mutation breeding of acetoin high producing Bacillus subtilis blocked in 2,3-butanediol dehydrogenase World Journal of Microbiology & Biotechnology. ,vol. 29, pp. 1783- 1789 ,(2013) , 10.1007/S11274-013-1339-8