Poor immunogenicity of BCG in helminth infected population is associated with increased in vitro TGF-β production

摘要: Abstract The only vaccine available against tuberculosis (TB), BCG, so effective in experimental animal models, has been under scrutiny for a long time owing to its variable efficacy pulmonary adults. In this study, we evaluated whether anti-helminthic therapy prior BCG vaccination could increase the immunogenicity of helminth infected population. We recruited volunteers with evidence mycobacterial infection and who were asymptomatic carriers helminths. subjects randomized receive either drugs or placebo. Three months later, was administered volunteers. Mycobacterial antigen-specific cytokine responses assessed 2 after vaccination. results show that peripheral blood mononuclear cells obtained from placebo group found have lower frequency IFN-γ (129 vs 191, p = 0.03) IL-12 (149 243, p = 0.013) producing per 2 × 105 PBMC (peripheral cells) when stimulated vitro antigen mixture (purified protein derivative (PPD)) compared those dewormed group. On other hand had higher TGF-β response PPD (152 81.3, p = 0.002) T cell mitogen concanavalin A (Con A) (210 157, p = 0.03). However, no detectable IL-4 IL-5 observed PPD. Comparable numbers both induced groups upon stimulation (IL-4 217 p = 0.08) (131 103, p = 0.14). data presented here demonstrate chronic worm reduces humans associated increased production but not enhanced Th2 immune response.