Molecular engineering of high affinity single-chain antibody fragment for endothelial targeting of proteins and nanocarriers in rodents and humans.

摘要: Endothelial cells (EC) represent an important target for pharmacologic intervention, given their central role in a wide variety of human pathophysiologic processes. Studies lab animal species have established that conjugation drugs and carriers with antibodies directed to surface targets like the Platelet Cell Adhesion Molecule-1 (PECAM-1, highly expressed endothelial transmembrane protein) help achieve specific therapeutic interventions ECs. To translate such "vascular immunotargeting" clinical practice, it is necessary replace by advanced ligands are more amenable use humans. We report molecular design single chain variable antibody fragment (scFv) binds high affinity PECAM-1 cross-reacts its counterpart rats other species, allowing parallel testing vivo microfluidic model. Site-specific modification scFv allows protein cargo liposomes, enabling targeting these models. This study provides template engineering ligands, studies drug subsequent