Two bacteriophage T4 base plate genes (25 and 26) and the DNA repair GeneuvsY belong to spatially and temporally overlapping transcription units

摘要: Abstract The bacteriophage T4 DNA recombination-repair geneuvsY located at or near an origin of replication and adjacent to the late base plate genes 25 26. Our present results reveal a complex transcription pattern in region encompassing these genes. Most significantly, uvsY two ORFs, downstream it, all which are transcribed from middle promoter before onset replication, also part larger unit includes 26 not only late, but early one several promoters further upstream. Translation, however, is inhibited by secondary structures sequester ribosome binding site transcript. We discuss possible advantages transcriptional patterns for recombination, repair. predicted andin vivo-expressed 23.9-kDa product gene smaller than reported size protein isolated plates, suggesting that nascent gp26 might be processed during assembly.