Molecular biology and biochemistry of pyruvate dehydrogenase complexes 1

摘要: In most organisms, the pyruvate dehydrogenase complex catalyzes pivotal irreversible reaction that leads to consumption of glucose in aerobic, energy-generating pathways. A combination biochemical and molecular biology studies have greatly expanded our understanding overall structural organization this multicomponent system, delineated locations elucidated functions domains catalytic components, revealed significant evolutionary changes. Important progress was deduction primary amino acid sequences from cDNA clones for each components several species. The greatest detail is available FAD-containing dihydrolipoamide component, which only component tertiary structure information has recently emerged. For acetyltransferase core a similar but species-variable multidomain established responsible distinct architectures inner cores, peripheral binding other conveyance intermediates between distantly separated active sites. second lipoyl-bearing protein X, been shown play critical role function many higher organisms. Although much known about means effector modulation mammalian activity, identification signal eliciting its regulation by insulin still poses an exciting challenge.