Human Peripheral Blood Mononuclear Cell Function and Dendritic Cell Differentiation Are Affected by Bisphenol-A Exposure

摘要: Environmental pollutants, including endocrine disruptor chemicals (EDCs), interfere on human health, leading to hormonal, immune and metabolic perturbations. Bisphenol-A (BPA), a main component of polycarbonate plastics, has been receiving increased attention due its worldwide distribution with large exposure. In humans, BPA, for estrogenic activity, may have role in autoimmunity, inflammatory allergic diseases. To this aim, we assessed the effect low BPA doses functionality peripheral blood mononuclear cells (PBMCs), vitro differentiation dendritic from monocytes (mDCs). Fresh samples were obtained 12 healthy adult volunteers. PBMCs left unstimulated or activated mitogen phytohemagglutinin (PHA) anti-CD3 anti-CD28 antibodies incubated presence absence at 0.1 1nM concentrations. The immune-modulatory was by evaluating cell proliferation levels interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-10 (IL-10) interleukin-13 (IL-13) secreted PBMCs. mDCs differentiated IL-4 GC-CSF without expression differentiation/maturation markers (CD11c, CD1a, CD86, HLA-DR) evaluated flow cytometry; furthermore, panel 27 different cytokines, growth factors chemokines assayed mDC culture supernatants. significantly upon exposure compared untreated cells. addition, significant decrease IL-10 secretion observed either mitogen-stimulated cells, both Similarly, IL-13 reduced, mainly antiCD3/CD28. By contrast, no changes IFN-γ production found any condition assayed. Finally, density expressing CD1a concomitantly decreased HLA-DR CD86 activation markers. conclusion, humans causes modulation proliferative capacity cytokine production, alteration phenotype. These alterations suggest that dose chronic could be involved deregulation possibly susceptibility develop autoimmune