Modulation of synaptic transmission by the voltage-gated potassium channel Eag1

摘要: The voltage-gated potassium channel Kv10.1 is widely expressed in the mammalian brain, but its function has not been described. Previous studies revealed highest expression levels hippocampus and cerebellum suggested a synaptic localization of channel. We studied role Kv10.1 in cerebellar physiology using whole-cell patch clamp 2-photon Ca2+ imaging Kv10.1-deficient mice wildtype controls. The excitability action potential shape granule cell bodies was unchanged despite high expression mRNA, providing functional evidence for synaptic localization neurons. Ca2+-influx into parallel fiber boutons was enhanced mutants in response to stimulation with 3 potentials, not after one pulse. Further, exhibited increased facilitation at the parallel C Purkinje synapse input frequencies. We propose that serves double presynapse. In the slow state, it is implicated controlling excitability, whereas repeated activity accelerates activation kinetics turn an important modulator action potential during repetitive high-frequency stimulation. Like this, prevent excessive excitation terminal when other channels suffer cumulative inactivation.