Isolation and characterisation of Chrysanthemum crassum SOS1, encoding a putative plasma membrane Na(+) /H(+) antiporter.

摘要: A full-length cDNA homologue of SOS1 (salt overly sensitive 1) was isolated from the salinity-tolerant species Chrysanthemum crassum and found to encode a Na(+) /H(+) antiporter, using degenerate PCR RACE-PCR. The 3752-bp sequence comprised 3438 bp open reading frame, encoding 127-kDa protein with 12 transmembrane domains within its N terminal portion, hydrophilic cytoplasmic tail in C-terminal portion. CcSOS1 appears be plasma membrane protein, shares ∼62% identity at peptide level Arabidopsis thaliana homologue. Expression roots C. salinity stress, while leaves down-regulated presence abscisic acid. transcript abundance reduced both plants exposed low temperature, it increased (but not roots) after drought stress. expression regulated CaCl2 . heterologous complementation assay yeast suggested that directs efflux, mimicking function endogenous NHA1 protein. Thus salinity-inducible antiporter. This gene may useful transgenic approaches improving tolerance related ornamental species.