Optical Waveguide Lightmode Spectroscopic Techniques for Investigating Membrane-Bound Ion Channel Activities
作者： Inna Székács , Nóra Kaszás , Pál Gróf , Katalin Erdélyi , István Szendrő
DOI: 10.1371/JOURNAL.PONE.0081398
关键词:
摘要: Optical waveguide lightmode spectroscopic (OWLS) techniques were probed for monitoring ion permeation through channels incorporated into artificial lipid environment. A novel sensor set-up was developed by depositing liposomes or cell-derived membrane fragments onto hydrophilic polytetrafluoroethylene (PTFE) membrane. The fibrous material of PTFE could entrap lipoid vesicles and the water-filled pores provided environment domains lipid-embedded proteins. surface kept clean from holder a water- ion-permeable polyethylene terephthalate (PET) mesh. tested with egg yolk lecithin containing gramicidin enriched in GABA-gated anion channels. method allowed move Na+ organic cations detecting Cl–-channel functions (α5β2γ2) GABAA receptor presence absence GABA competitive GABA-blocker bicuculline.
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Bryndis Birnir, Mansoureh Eghbali, GB Cox, PW Gage, None, GABA concentration sets the conductance of delayed GABAA channels in outside-out patches from rat hippocampal neurons. The Journal of Membrane Biology. ,vol. 181, pp. 171- 183 ,(2001) , 10.1007/S00232-001-0021-5
Surface plasmon resonance based sensors Surface Plasmon Resonance Based Sensors. ,vol. 5, pp. 51- 51 ,(2006) , 10.1007/B100321
Handbook of Biosensors and Biochips. University of Nairobi. ,(2008) , 10.1002/9780470061565
Magnus Brändén, Seyed R. Tabaei, Gerhard Fischer, Richard Neutze, Fredrik Höök, Refractive-Index-Based Screening of Membrane-Protein-Mediated Transfer across Biological Membranes Biophysical Journal. ,vol. 99, pp. 124- 133 ,(2010) , 10.1016/J.BPJ.2010.03.059
J Vörös, JJ Ramsden, G Csucs, I Szendrő, SM De Paul, M Textor, ND Spencer, Optical grating coupler biosensors. Biomaterials. ,vol. 23, pp. 3699- 3710 ,(2002) , 10.1016/S0142-9612(02)00103-5
K. Cottier, R. Horvath, Imageless microscopy of surface patterns using optical waveguides Applied Physics B. ,vol. 91, pp. 319- 327 ,(2008) , 10.1007/S00340-008-2994-6
Kaori Sugihara, János Vörös, Tomaso Zambelli, A gigaseal obtained with a self-assembled long-lifetime lipid bilayer on a single polyelectrolyte multilayer-filled nanopore. ACS Nano. ,vol. 4, pp. 5047- 5054 ,(2010) , 10.1021/NN100773Q
Thai Phung, Yanli Zhang, James Dunlop, Julie Dalziel, Bilayer lipid membranes supported on Teflon filters: a functional environment for ion channels. Biosensors and Bioelectronics. ,vol. 26, pp. 3127- 3135 ,(2011) , 10.1016/J.BIOS.2010.12.013
L. Steller, M. Kreir, R. Salzer, Natural and artificial ion channels for biosensing platforms Analytical and Bioanalytical Chemistry. ,vol. 402, pp. 209- 230 ,(2012) , 10.1007/S00216-011-5517-Y
N. Trummer, N. Adányi, M. Váradi, I. Szendrö, Modification of the surface of integrated optical wave-guide sensors for immunosensor applications. Fresenius Journal of Analytical Chemistry. ,vol. 371, pp. 21- 24 ,(2001) , 10.1007/S002160100929