On the localization of certain mitochondrial and microsomal enzymes in rat liver nuclei

摘要: Abstract 1. Nuclear fractions were prepared from rat liver homogenates by means of aqueous procedures utilizing 0–25 M sucrose solutions, 2.2–2.3 or 0.25 solutions containing Triton X-100. 2. The activities the following enzymes determined in each these nuclear preparations: glutamate dehydrogenase, glutamine synthetase, nicotinamide adenine dinudeotidase, succinate-cytochrome c reductase, 5'-nucleotidase, glucose-6-phosphatase, glutamate-pynivate transaminase, hexokinase, NADH oxidase, and lactamase. 3. Nuclei puofied treatment with X-100 contained relatively firmly bound synthetase at specific 3-6-fold that original homogenate. 4. This purification as judged recovery DNA enzyme activity was conditioned species concentration divalent cation present homogenizing medium. 5. possible significance findings is discussed.