An inhaled tumor necrosis factor‐alpha‐derived TIP peptide improves the pulmonary function in experimental lung injury

摘要: INTRODUCTION The lectin-like domain of TNF-α enhances the fluid clearance across alveolar barrier. For experimental purposes, can be mimicked by a synthetic peptide representing TIP-motif TNF-α. present study aims to assess acute effect TIP on pulmonary function in porcine model respiratory distress syndrome (ARDS). METHODS Lung injury was induced 16 pigs (25-27 kg) bronchoalveolar lavage followed injurious ventilation. Following randomisation, either nebulised (1 mg/kg; AP301, APEPTICO, Vienna, Austria) or water for injection (control group) administered. During 5 h monitoring, extravascular lung index (EVLWI), quotient partial pressure oxygen and inspired concentration (PaO(2) /FiO(2) ) shunt fraction were repetitively assessed. data evaluated an analysis variance including Bonferroni-Holm correction. RESULTS Comparable baseline conditions both groups achieved. Ventilatory parameters standardised groups. In group, significant reduction EVLWI simultaneous increase PaO(2) ratio shown (each P < 0.0001). No changes control group observed (EVLWI: P = 0.43, : P = 0.60). intergroup comparison demonstrates advantage inhalation over placebo P < 0.0001, P = 0.004, fraction: P = 0.0005). CONCLUSIONS induces amelioration clinical surrogate model. By mimicking domain, AP301 is innovative approach as supportive therapy ARDS.