Growth inhibition of human endothelial cells by human recombinant tumor necrosis factor alpha and interferon-gamma.
作者： Thekla Mauerhoff , Antonino Belfiore , Ricardo Pujol-Borrell , Gian Franco Bottazzo
DOI: 10.1177/030089169408000412
关键词:
摘要: We investigated the effect of recombinant tumor necrosis factor-alpha (rTNF-alpha) on proliferative response human umbilical vein endothelial cells (HUVEC) to normal serum (NHS), in absence or presence interferon (IFN)-gamma. rTNF-alpha significantly impaired NHS-stimulated HUVEC growth at a dose as low 0.1 U/ml. The inhibitory was dose-dependent up 50-100 U/ml and already evident after 2 h incubation. Doses range 10 completely suppressed 3H-thymidine uptake stimulated by 7.5% NHS, partially overcome 10-20% NHS. not cytotoxic doses 1000 rIFN-gamma also effective suppressing incorporation, (0.1 U/ml) rTFN-alpha showed an additive effect. TFN-alpha IFN-gamma antagonizing vascular endothelium variety factors contained could be relevant pathologic conditions involving damage proliferation.
sci-hub.se 参考文章(21)
Vito Ruggiero, Jan Tavernier, Walter Fiers, Corrado Baglioni, Induction of the synthesis of tumor necrosis factor receptors by interferon-gamma. Journal of Immunology. ,vol. 136, pp. 2445- 2450 ,(1986)
Jordan S Pober, Alan H Stolpen, Walter Fiers, Eva C Guinan, Recombinant tumor necrosis factor and immune interferon act singly and in combination to reorganize human vascular endothelial cell monolayers. American Journal of Pathology. ,vol. 123, pp. 16- 24 ,(1986)
Actions of Tumor Necrosis Factor on Cultured Vascular Endothelial Cells: Morphologic Modulation, Growth Inhibition, and Cytotoxicity Journal of the National Cancer Institute. ,vol. 76, pp. 1113- 1121 ,(1986) , 10.1093/JNCI/76.6.1113
R Moser, B Schleiffenbaum, P Groscurth, J Fehr, Interleukin 1 and tumor necrosis factor stimulate human vascular endothelial cells to promote transendothelial neutrophil passage. Journal of Clinical Investigation. ,vol. 83, pp. 444- 455 ,(1989) , 10.1172/JCI113903
Michael P Bevilacqua, Jordan S Pober, Gerard R Majeau, Walter Fiers, Ramzi S Cotran, Michael A Gimbrone Jr, None, Recombinant tumor necrosis factor induces procoagulant activity in cultured human vascular endothelium: characterization and comparison with the actions of interleukin 1 Proceedings of the National Academy of Sciences of the United States of America. ,vol. 83, pp. 4533- 4537 ,(1986) , 10.1073/PNAS.83.12.4533
S. Joseph Leibovich, Peter J. Polverini, H. Michael Shepard, David M. Wiseman, Vera Shively, Nureddin Nuseir, Macrophage-induced angiogenesis is mediated by tumour necrosis factor- α Nature. ,vol. 329, pp. 630- 632 ,(1987) , 10.1038/329630A0
Lothar Schweigerer, Barbara Malerstein, Denis Gospodarowicz, Tumor necrosis factor inhibits the proliferation of cultured capillary endothelial cells Biochemical and Biophysical Research Communications. ,vol. 143, pp. 997- 1004 ,(1987) , 10.1016/0006-291X(87)90350-0
M. Frater-Schroder, W. Risau, R. Hallmann, P. Gautschi, P. Bohlen, Tumor necrosis factor type alpha, a potent inhibitor of endothelial cell growth in vitro, is angiogenic in vivo. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 84, pp. 5277- 5281 ,(1987) , 10.1073/PNAS.84.15.5277
T. Collins, L. A. Lapierre, W. Fiers, J. L. Strominger, J. S. Pober, Recombinant human tumor necrosis factor increases mRNA levels and surface expression of HLA-A,B antigens in vascular endothelial cells and dermal fibroblasts in vitro Proceedings of the National Academy of Sciences of the United States of America. ,vol. 83, pp. 446- 450 ,(1986) , 10.1073/PNAS.83.2.446
V. C. Broudy, K. Kaushansky, G. M. Segal, J. M. Harlan, J. W. Adamson, Tumor necrosis factor type alpha stimulates human endothelial cells to produce granulocyte/macrophage colony-stimulating factor Proceedings of the National Academy of Sciences of the United States of America. ,vol. 83, pp. 7467- 7471 ,(1986) , 10.1073/PNAS.83.19.7467