Measuring the regulation of dynein activity during flagellar motility

摘要: Abstract Flagellar and ciliary motility are driven by the activity of dynein, which produces microtubule sliding within axonemes. Our goal is to understand how dynein motile regulated produce characteristic oscillatory movement flagella. Analysis various parameters, such as frequency shear angle in beating flagella, important for understanding time-dependent changes amounts along flagellum. Demembranated flagella can be reactivated a wide range ATP concentrations (from 2 μ M several m ) beat increases with an increase ATP. By imposed vibration micropipette that caught sperm head suction, however, motion modulated so synchronize over 20–70 Hz at 2 m The time-averaged velocity calculated product decreases when below undriven flagellar frequency, but higher frequencies, it remains constant. In addition role ATP, mechanical force bending involved activation dynein. elastase-treated axonemes, bending-dependent regulation achieved. This chapter provides overview approaches, using sea urchin studying measurements or without force.