Escherichia coli and Candida albicans Induced Macrophage Extracellular Trap-Like Structures with Limited Microbicidal Activity

摘要: The formation of extracellular traps (ETs) has recently been recognized as a novel defense mechanism in several types innate immune cells. It suggested that these structures are toxic to microbes and contribute significantly killing pathogens. However, the role ETs formed by macrophages (METs) against remains little known. In this study, we demonstrated subset murine J774A.1 macrophage cell line (8% 17%) peritoneal (8.5% 15%) form METs-like (METs-LS) response Escherichia coli Candida albicans challenge. We found only portion METs-LS, which released dying macrophages, showed detectable effects on trapped E. but not C. albicans. Fluorescence scanning electron microscopy analyses revealed that, vitro, both microorganisms were entrapped METs-LS composed DNA microbicidal proteins such histone, myeloperoxidase lysozyme. components nucleus mitochondrion origins structures. Additionally, occurred independently ROS produced NADPH oxidase, process did result lysis. summary, our results emphasized induced cells activity differs greatly. propose function is contain invading at infection site, thereby preventing systemic diffusion them, rather than them.