CLOCK promotes 3T3-L1 cell proliferation via Wnt signaling

摘要: Circadian genes control most of the physiological functions including cell cycle. Cell proliferation is a critical factor in differentiation progenitor cells. However, role Clock gene regulation cycle via wingless-type (Wnt) pathway and relationship between adipogenesis are unclear. We found that circadian locomotor output cycles kaput (Clock) regulated 3T3-L1 preadipocytes. attenuation inhibited viability preadipocytes counting kit 8. The expression c-Myc Cyclin D1 decreased dramatically when was silenced with short interfering RNA also fat tissue adipose tissue-derived stem cells Clock(Δ19) mice. directly controls components Wnt signal transduction pathway, which verified by serum shock, chromatin immunoprecipitation, Western blot, quantitative real-time polymerase chain reaction (qRT-PCR). Furthermore, IWR-1, inhibitor, promotion CLOCK, detected assay, flow cytometry, qRT-PCR. Therefore, CLOCK transcription signaling promotes progression on day 2 Oil Red O staining qRT-PCR detection probably related to cellular differentiation. These data provide evidence regulates affects adipogenesis. © 2016 IUBMB Life, 68(7):557-568, 2016.