Several dinucleoside polyphosphates are acceptor substrates in the T4 RNA ligase catalyzed reaction
作者： Eva A. Atencia , María Montes , María A. Günther Sillero , Antonio Sillero
DOI: 10.1046/J.1432-1327.2000.01165.X
关键词:
摘要: Several dinucleoside polyphosphates accept cytidine-3', 5'-bisphosphate from the adenylylated donor 5'-adenylylated cytidine 5',3'-bisphosphate in T4 RNA ligase catalyzed reaction. The synthesized a first step, ATP and cytidine-3',5'-bisphosphate, is used as substrate to transfer cytidine-3',5'-bisphosphate residue 3'-OH group(s) of diguanosine tetraphosphate (Gp4G) giving rise Gp4GpCp pCpGp4GpCp ratio approximately 10 : 1, respectively. was characterized by treatment with snake venom phosphodiesterase alkaline phosphatase analysis (chromatographic position UV spectra) reaction products HPLC. apparent Km values measured for Gp4G this were 4 mM 0.4 mM, In presence 0.5 relative efficiencies following nucleoside(5')oligophospho(5')nucleosides acceptors 5', 3'-bisphosphate are indicated parentheses: (100); Gp5G (101); Ap4G (47); Ap4A (39). Gp2G, Gp3G Xp4X not substrates Dinucleotides containing two guanines at least four inner phosphates preferred their group(s).
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