Laminin α5 modulates fibroblast proliferation in epidural fibrosis through the PI3K/AKT/mTOR signaling pathway

摘要: Lumbar laminectomy is commonly deemed as the most valid surgery for a series of lumbar illnesses, such disc herniation, which could lead to spinal canal stenosis. However, epidural fibrosis one common complications that limits application laminectomy, mainly caused by proliferation local fibroblasts. Laminins are glycoproteins consist α, β and γ chains, serve crucial role in biological cell behaviors, adhesion, differentiation, migration proliferation, especially isoform with fifth α chain-laminin α5. The PI3K/AKT/mTOR signaling pathway was demonstrated be associated various functions cells. aim present study explore whether laminin α5 an important factor modulating fibroblasts through activation pathway. In animal model, results hematoxylin-eosin staining, counting, Masson's trichrome staining immunohistochemical showed positively fibrosis. Furthermore, verify assumption modulate fibroblast signal pathway, were transfected α5-small interfering (si)RNA. western blotting (proliferating nuclear antigen cyclin D1), Cell Counting Kit-8 EdU incorporation assays indicated proliferative level decreased, expression phosphorylated (p)-focal adhesion kinase 1, p-AKT p-mTOR reduced. Subsequently, overexpressed change proteins contrasted observed siRNA. interfere Finally, inhibition LY294002 resulted decreased proliferation. conclusion,