Assessment of the Bemisia tabaci CYP6CM1vQ transcript and protein levels in laboratory and field-derived imidacloprid-resistant insects and cross-metabolism potential of the recombinant enzyme

摘要: Over-expression of the cytochrome P450 CYP6CM1 gene has been associated with imidacloprid resistance in a number Q and B biotype Bemisia tabaci laboratory strains from distinct geographical origins worldwide. We recently demonstrated that version protein (CYP6CM1vQ) is capable metabolizing imidacloprid. Here, we show levels BtCYP6CM1vQ were also elevated laboratory-resistant field-derived populations, variable levels, collected Crete. High CYP6CM1vQ transcripts determined survivors heterogeneous field population, after exposure to discriminating dosage. Using peptide antibody-based detection assays, line transcriptional data, higher imidacloprid-resistant insects, which further implicates as causal factor resistance. Finally, assessment cross-metabolism potential against additional neonicotinoid molecules used for B. control revealed clothianidin thiacloprid, but not acetamiprid or thiamethoxam, are metabolized by recombinant enzyme vitro.