Acetogenin Extracted from Annona muricata Prevented the Actions of EGF in PA-1 Ovarian Cancer Cells.

摘要: Background In individuals with ovarian cancer, an increase in the circulating level of epidermal growth factor (EGF) is readily apparent. Ovarian cancer cells exhibit signaling pathway (EGFR) and respond to EGF. Annona Muricata (AM) has been shown decrease cell proliferation however, role AM regulating EGF actions not yet be reported. Objective this study, we proposed that fractionated compound acetogenin can inhibit activation EGFRregulated cascades such as MAPK7 / PI3K-Akt mTOR STAT upon stimulation. Methods Ethanolic extract was prepared for whole plant Thin Layer Chromatography (TLC) performed characterize secondary metabolites each fraction assessed using kedde reagent presence acetogenin. The effects acetogenins were then tested on survival PA-1 under basal stimulated conditions. To delineate EGFR cascades, silico docking studies conducted. Results decreased viability induced indicating inhibitory specifically illustrated when binding TK RU which subsequently resulted a reduction cells. Discussion vital regulatory reported study indicate significant anticancer activities from AM. function predicted it binds Asp837 (phosphoracceptor site) EGFR, essential phosphorylation substrates domain promote downstream signaling. Conclusion This isolated effectively inhibited through impaired