Standardized determination of real‐time PCR efficiency from a single reaction set‐up

摘要: We propose a computing method for the estimation of real-time PCR amplification efficiency. It is based on statistic delimitation beginning exponentially behaving observations in kinetics. ground fluorescence phase, nonexponential and plateau phase were excluded from calculation process by separate mathematical algorithms. validated experimental data multiple targets obtained LightCycler platform. The developed yields results higher accuracy than currently used serial dilutions efficiency estimation. single reaction set-up sensitive to differences starting concentrations target sequence samples. Furthermore, it resists subjective influence researchers, can therefore be fully instrumentalized.