Modulation of allogeneic stimulation in man. I. Characterization of an in vitro induced suppressor macrophage population.

摘要: Cultured human peripheral blood mononuclear cells suppressed the allogeneic response of fresh autologous lymphocytes. This suppressor activity developed gradually over a period one week. The primarily responsible for this effect were enriched by Ficoll density gradient centrifugation. It was found that cell is large, low nylon wool adherent, radioresistant, phagocytic, and nonspecific esterase positive cell. Moreover, these did not form E rosettes Fc positive. Electron microscopy confirmed macrophage like. Suppressor due to cytotoxicity, crowding, or steric hinderance cultured cells. population appear inhibit via prostaglandin arginase release, interfere with tritiated thymidine uptake release endogenous thymidine. above system viewed as an in vitro model immune regulation macrophages, context response.