One-pot synthesis of glutathione by a two-enzyme cascade using a thermophilic ATP regeneration system
作者： Xing Zhang , Hui Wu , Bing Huang , Zhimin Li , Qin Ye
DOI: 10.1016/J.JBIOTEC.2016.11.034
关键词:
摘要: In vitro cascade catalysis using enzyme-based system is becoming a promising biomanufacturing platform for biofuels and biochemicals production. Glutathione pivotal non-protein thiol compound widely applied in food pharmaceutical industries. this study, glutathione was synthesized by bifunctional synthetase together with thermophilic ATP regeneration through two-enzyme vitro. Four synthetases from Streptococcus sanguinis, S. gordonii, uberis Bacillus cereus were synthesis. The sanguinis selected coupled the polyphosphate kinase Thermosynechococcus elongatus BP-1 regenerating to produce one pot. optimized system, 28.5mM produced within 5h due efficient low-cost polyphosphate. yield based on added l-cysteine reached 81.4% productivity of achieved 5.7mM/h. one-pot indicated potential biotransformation industrial production glutathione.
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