Oligonucleotide-based array CGH as a diagnostic tool in multiple myeloma patients

摘要: Multiple myeloma (MM) is a hematological disease caused by malignant proliferation of clonal plasma cells (PCs) known for its clinical and biological heterogeneity. Identification chromosomal changes in genome PCs plays key role MM pathogenesis supposed to have important prognostic significance patients. There are two major genetic entities MM. Hyperdiploid tumors (H-MM), which include about 50% tumors, often multiple trisomies involving chromosomes 3, 5, 7, 9, 11, 15, 19, 21 substantially lower prevalence IgH translocations. Nearly half non-hyperdiploid (NH-MM), mostly one five recurrent translocations: 11ql13 (CCND1), 6p21 (CCND3), 16q23 (MAF), 20q12 (MAFB), 4p16 (FGFR3 MMSET). The development expanded use new technologies, such as genome-wide array-based comparative genomic hybridization (aCGH) has accelerated research This technique powerful tool globally analyze copy number tumor single reaction study cancer biology behaviors. It widely overcame routinely used cytogenetic techniques (G-banding, FISH) both minimal resolution amount obtained data further analyses applications. Array CGH now better understanding molecular phenotypes, sensitivity particular chemotherapeutic agents, prognosis these diseases. paper brings brief literature methodic overview oligonucleotide-based array-CGH diagnosis.