T cell-induced Ig allotypic suppression in mice. II. Both CD4+ CD8- and CD4- CD8+ T cell subsets from sensitized Igha mice are required to induce suppression of Igh-1b allotype expression.

摘要: We established the phenotype of T splenocytes (Ts) from Igha/a BALB/c mice sensitized against B Ighb/b CB20 congenic that induce Igh-1b (IgG2a Ighb haplotype) suppression. This was achieved by studying action anti-T cell subset mAb on capacity Ts to this chronic allotypic suppression in Igha/b (BALB/c x CB20)F1 mice. The were treated with cytotoxic anti-mouse CD4 or CD8 rat vitro before their injection into newborns vivo after newborns. Exposure either anti-CD8 anti-CD4 leads loss Mixing CD4+-cell-depleted and CD8+-cell-depleted preparations restored cells Thus, both CD4+ CD8- CD4- CD8+ are required for induction Bone marrow Igh-1b-suppressed adult shown be able durably express when transferred irradiated hosts whereas whole spleen such donors failed do it. Abrogation treatment heterozygotes but a lower efficiency than homozygotes, all being chronically suppressed. population essential maintaining is resistant 600 rad irradiation seems slightly inhibited administration free Igh-1b.