pH-switchable bioelectrocatalysis based on layer-by-layer films assembled through specific boronic acid-diol recognition

摘要: Abstract Phenylboronic acid (PBA) moieties are grafted onto the backbone of poly(acrylic acid) (PAA), forming PAA-PBA polyelectrolyte. The and polysaccharide dextran (Dex) then assembled layer-by-layer (LbL) into {PAA-PBA/Dex} n films on electrode surface through boronic acid-diol specific recognition between them. cyclic voltammetric response ferrocenedicarboxylic (Fc(COOH) 2 ) at film electrodes is sensitive to environmental pH. At pH 5.5, CV peak currents quite large, “on” state; 8.0, however, significantly suppressed with “off” state. This pH-sensitive on–off property toward probe can be used switch bioelectrocatalysis glucose mediated by Fc(COOH) in present oxidase (GOD) solution changing surrounding Furthermore, glycoenzyme GOD also {PAA-PBA/GOD} LbL interaction them, so that immobilization enzyme realized. show behavior as mediator. model system may open a new way establish foundation for fabricating pH-responsive biosensors based enzymatic electrocatalysis.