The E Site and Its Importance for Improving Accuracy and Preventing Frameshifts

摘要: The ribosome contains three tRNA binding sites, the A, P, and E sites. Although site is separated from A via intervening P site, there striking communication between these This cross-talk plays an important role for accuracy of decoding process. Codon–anticodon interaction at seems to be signal switch into post-translocational (POST) state characterized by a low affinity site. low-affinity forces ternary complexes aminoacyl-tRNA•EF-Tu•GTP enter center preventing selection non-cognate aminoacyl-tRNAs incorporation incorrect amino acid. has consequence that only 1 in 400 misincorporations affects protein function. Another aspect allostery sites during elongation are always least two tRNAs present on same time. Since firmly bound whereas mRNA held predominantly codon–anticodon interaction, it movement translocation pulls through ribosome. In fact, six base pairs adjacent interactions instrumental maintaining reading frame, evidence without E-tRNA frame would lost after about 50 acids nascent chain.