Rare biosphere exploration using high-throughput sequencing: research progress and perspectives

摘要: Identification of rare species and mapping their distributions is crucial for understanding natural causes consequences accelerating declines. However, detection in both terrestrial especially aquatic communities typically dominated by numerous microscopic (i.e. biosphere) represents a formidable technical challenge. Rapid advances high-throughput sequencing (HTS) technologies have revolutionized biodiversity studies the biosphere, also stimulated associated debates. Here we summarize research progress, discuss debates problems, propose possible solutions future to address these issues. In addition, provide take-home messages experimental design data interpretation when utilizing HTS techniques biosphere exploration ecology conservation biology.