Active Rumen Bacterial and Protozoal Communities Revealed by RNA-Based Amplicon Sequencing on Dairy Cows Fed Different Diets at Three Physiological Stages.
作者： Barbara Cardazzo , Lucia Bailoni , Lisa Carraro , Marco Cardin
DOI: 10.3390/MICROORGANISMS9040754
关键词:
摘要: Seven Italian Simmental cows were monitored during three different physiological stages, namely late lactation (LL), dry period (DP), and postpartum (PP), to evaluate modifications in their metabolically-active rumen bacterial protozoal communities using the RNA-based amplicon sequencing method. The community was dominated by seven phyla: Proteobacteria, Bacteroidetes, Firmicutes, Spirochaetes, Fibrobacteres, Verrucomicrobia, Tenericutes. relative abundance of phylum Proteobacteria decreased from 47.60 28.15% LL DP then increased 33.24% PP. An opposite pattern LL, DP, PP stages observed for phyla Verrucomicrobia (from 0.96 4.30 1.69%), Elusimicrobia 0.32 2.84 0.25%), SR1 0.50 2.08 0.79%). families Succinivibrionaceae Prevotellaceae while Ruminococcaceae increased. Bacterial genera Prevotella Treponema least abundant as compared PP, Ruminobacter Succinimonas most DP. eukaryotic Ciliophora, which showed a significant decrease 97.6 93.9 92.6 respectively. In conclusion, stage-dependent dietary changes resulted clear shift microbial communities.
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S. K. R. Karnati, Z. Yu, J. T. Sylvester, B. A. Dehority, M. Morrison, J. L. Firkins, Technical note: Specific PCR amplification of protozoal 18S rDNA sequences from DNA extracted from ruminal samples of cows Journal of Animal Science. ,vol. 81, pp. 812- 815 ,(2003) , 10.2527/2003.813812X
E Pers-Kamczyc, P Zmora, A Cieslak, M Szumacher-Strabel, None, Development of nucleic acid based techniques and possibilities of their application to rumen microbial ecology research. Journal of Animal and Feed Sciences. ,vol. 20, pp. 315- 337 ,(2011) , 10.22358/JAFS/66189/2016
Cesar E. Guzman, Lara T. Bereza-Malcolm, Bert De Groef, Ashley E. Franks, Presence of Selected Methanogens, Fibrolytic Bacteria, and Proteobacteria in the Gastrointestinal Tract of Neonatal Dairy Calves from Birth to 72 Hours PLOS ONE. ,vol. 10, pp. e0133048- ,(2015) , 10.1371/JOURNAL.PONE.0133048
Matthew Sean McCabe, Paul Cormican, Kate Keogh, Aaron O’Connor, Eoin O’Hara, Rafael Alejandro Palladino, David Anthony Kenny, Sinéad Mary Waters, None, Illumina MiSeq Phylogenetic Amplicon Sequencing Shows a Large Reduction of an Uncharacterised Succinivibrionaceae and an Increase of the Methanobrevibacter gottschalkii Clade in Feed Restricted Cattle PLOS ONE. ,vol. 10, pp. e0133234- ,(2015) , 10.1371/JOURNAL.PONE.0133234
Eric Gaidos, Antje Rusch, Melissa Ilardo, Ribosomal tag pyrosequencing of DNA and RNA from benthic coral reef microbiota: community spatial structure, rare members and nitrogen-cycling guilds. Environmental Microbiology. ,vol. 13, pp. 1138- 1152 ,(2011) , 10.1111/J.1462-2920.2010.02392.X
Gemma Henderson, Faith Cox, Siva Ganesh, Arjan Jonker, Wayne Young, Leticia Abecia, Erika Angarita, Paula Aravena, Graciela Nora Arenas, Claudia Ariza, Graeme T Attwood, Jose Mauricio Avila, Jorge Avila-Stagno, André Bannink, Rolando Barahona, Mariano Batistotti, Mads F Bertelsen, Aya Brown-Kav, Andres M Carvajal, Laura Cersosimo, Alexandre Vieira Chaves, John Church, Nicholas Clipson, Mario A Cobos-Peralta, Adrian L Cookson, Silvio Cravero, Omar Cristobal Carballo, Katie Crosley, Gustavo Cruz, María Cerón Cucchi, Rodrigo de la Barra, Alexandre B De Menezes, Edenio Detmann, Kasper Dieho, Jan Dijkstra, William LS dos Reis, Mike ER Dugan, Seyed Hadi Ebrahimi, Emma Eythórsdóttir, Fabian Nde Fon, Martín Fraga, Francisco Franco, Chris Friedeman, Naoki Fukuma, Dragana Gagić, Isabelle Gangnat, Diego Javier Grilli, Vahideh Heidarian Miri, Emma Hernandez-Sanabria, Alma Ximena Ibarra Gomez, Olubukola A Isah, Suzanne Ishaq, Elie Jami, Juan Jelincic, Juha Kantanen, William J Kelly, Seon-Ho Kim, Athol Klieve, Yasuo Kobayashi, Satoshi Koike, Jan Kopecny, Torsten Nygaard Kristensen, Sophie Julie Krizsan, Hannah LaChance, Medora Lachman, William R Lamberson, Suzanne Lambie, Jan Lassen, Sinead C Leahy, Sang-Suk Lee, Florian Leiber, Eva Lewis, Bo Lin, Raúl Lira, Peter Lund, Edgar Macipe, Lovelia L Mamuad, Hilário Cuquetto Mantovani, Gisela Ariana Marcoppido, Cristian Márquez, Cécile Martin, Gonzalo Martinez, Maria Eugenia Martinez, Olga Lucía Mayorga, Tim A McAllister, Chris McSweeney, Lorena Mestre, Elena Minnee, Makoto Mitsumori, Itzhak Mizrahi, Isabel Molina, Andreas Muenger, Camila Muñoz, Bostjan Murovec, John Newbold, Victor Nsereko, Michael O’Donovan, Sunday Okunade, Brendan O’Neill, Sonia Ospina, Diane Ouwerkerk, Diana Parra, Luiz Gustavo Ribeiro Pereira, Cesar Pinares-Patiño, Phil B Pope, Morten Poulsen, Markus Rodehutscord, Tatiana Rodriguez, Kunihiko Saito, Francisco Sales, Catherine Sauer, Kevin Shingfield, Noriaki Shoji, Jiri Simunek, Zorica Stojanović-Radić, Blaz Stres, Xuezhao Sun, Jeffery Swartz, Zhi Liang Tan, Ilma Tapio, Tasia M Taxis, Nigel Tomkins, Emilio Ungerfeld, Reza Valizadeh, Peter van Adrichem, Jonathan Van Hamme, Woulter Van Hoven, Garry Waghorn, R John Wallace, Min Wang, Sinéad M Waters, Kate Keogh, Maren Witzig, Andre-Denis G Wright, Hidehisa Yamano, Tianhai Yan, David R Yáñez-Ruiz, Carl J Yeoman, Ricardo Zambrano, Johanna Zeitz, Mi Zhou, Hua Wei Zhou, Cai Xia Zou, Pablo Zunino, Peter H Janssen, Global Rumen Census Collaborators, Rumen microbial community composition varies with diet and host, but a core microbiome is found across a wide geographical range Scientific Reports. ,vol. 5, pp. 19175- 19175 ,(2015) , 10.1038/SREP14567
Liping Zhao, Qingxiang Meng, Liping Ren, Wei Liu, Xinzhuang Zhang, Yunlong Huo, Zhenming Zhou, Effects of Nitrate Addition on Rumen Fermentation, Bacterial Biodiversity and Abundance. Asian-australasian Journal of Animal Sciences. ,vol. 28, pp. 1433- 1441 ,(2015) , 10.5713/AJAS.15.0091
C. Benchaar, F. Hassanat, R. Gervais, P.Y. Chouinard, H.V. Petit, D.I. Massé, Methane production, digestion, ruminal fermentation, nitrogen balance, and milk production of cows fed corn silage- or barley silage-based diets. Journal of Dairy Science. ,vol. 97, pp. 961- 974 ,(2014) , 10.3168/JDS.2013-7122
G.T. Attwood, K. Reilly, Identification of proteolytic rumen bacteria isolated from New Zealand cattle Journal of Applied Bacteriology. ,vol. 79, pp. 22- 29 ,(1995) , 10.1111/J.1365-2672.1995.TB03119.X
BURK A. DEHORITY, AGNES A. ODENYO, Influence of Diet on the Rumen Protozoal Fauna of Indigenous African Wild Ruminants Journal of Eukaryotic Microbiology. ,vol. 50, pp. 220- 223 ,(2003) , 10.1111/J.1550-7408.2003.TB00121.X