Two putative-aquaporin genes are differentially expressed during arbuscular mycorrhizal symbiosis in Lotus japonicus

摘要: Arbuscular mycorrhizas (AM) are widespread symbioses that provide great advantages to the plant, improving its nutritional status and allowing fungus complete life cycle. Nevertheless, molecular mechanisms lead development of AM symbiosis not yet fully deciphered. Here, we have focused on two putative aquaporin genes, LjNIP1 LjXIP1, which resulted be upregulated in a transcriptomic analysis performed mycorrhizal roots Lotus japonicus. A phylogenetic has shown aquaporins belong different functional families: NIPs XIPs. Transcriptomic experiments independence their expression from but also close correlation with rhizobial interaction. Further transcript quantification revealed good between one them, LjNIP1, LjPT4, phosphate transporter is considered marker gene for functionality. By using laser microdissection, demonstrated expressed exclusively arbuscule-containing cells. agreement role as an aquaporin, capable transferring water when yeast protoplasts. Confocal eGFP-LjNIP1, under endogenous promoter, accumulates inner membrane system arbusculated Overall, results functionality specificity mycorrhiza-inducible L. One can novel at developmental stages arbuscule. At same time, LjXIP1 first XIP family transcriptionally regulated during symbiosis.