Production of tannase from wood-degrading fungus using as substrate plant residues: purification and characterization

摘要: In the present study Lenzites elegans, Schizophyllum commune, Ganoderma applanatum and Pycnoporus sanguineus (wood-degrading fungi) were assayed for their tannase producing potential in culture media containing plant residues or/and tannic acid as carbon source. Aspergillus niger was used positive control production. We also carried out isolation, purification characterization of enzyme from fungi selected major productor. The highest fungal growth observed A. L. elegans acid + glucose + plant (Fabiana densa). reached extracellular production a medium acid + F. densa supplemented with glucose + tannic densa. produced by purified DEAE-Sepharose. Km value 5.5 mM relative molecular mass about 163,000. Tannase stable at pH range 3.0–6.0 its optimum 5.5. showed an temperature 60°C between 40 60°C. This paper is first communication wood-degrading fungi. Fermentation technology to produce using xylophagous could be very important order take advantage industrial waste.