Mitochondrial respiration in human viable platelets-Methodology and influence of gender, age and storage.

摘要: Studying whole cell preparations with intact mitochondria and respiratory complexes has a clear benefit compared to isolated or disrupted due the dynamic interplay between other cellular compartments. Platelet have potential serve as source of human viable when studying mitochondrial physiology pathogenic mechanisms, well for diagnostics diseases. The objective present study was perform detailed evaluation platelet respiration using high-resolution respirometry. Further, we aimed explore limits sample size impact storage establish wide range reference data from different pediatric adult cohorts. Our results indicate that are suited ex-vivo analysis need minute amounts excellent reproducibility stability.