Cloning and characterization of spliced variants of the porcine G protein coupled receptor 120.
作者： Tongxing Song , Jie Peng , Jiao Ren , Hong-kui Wei , Jian Peng
DOI: 10.1155/2015/813816
关键词:
摘要: The polyunsaturated fatty acids (PUFAs) receptor GPR120 exerts a significant impact on systemic nutrient homeostasis in human and rodents. However, the porcine (pGPR120) has not been well characterized. In current study, we found that pGPR120 had 3 spliced variants. Transcript 1 encoded 362-amino (aa) wild type pGPR120-WT, which shared 88% homology with short form GPR120. was mainly expressed transcript of pGPR120. It predominantly ileum, jejunum, duodenum, spleen, adipose. (coding 320-aa isoform) detected while 2 310-aa only slightly spleen. A selective agonist for (TUG-891) PUFAs activated SRE-luc NFAT-luc reporter HEK293T cells transfected construct pGPR120-WT but pGPR120-V2. isoform dominant negative isoform. extracellular signal-regulated kinase 1/2 (ERK1/2) phosphorylation levels were by PUFAs, especially n-3 PUFA. These results showed although transcripts, transcript. TUG-891 PUFA, pGPR120-WT. study contributed to dissecting molecular regulation mechanisms PUFA pigs.
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Xiu-Lei Mo, Hong-Kui Wei, Jian Peng, Ya-Xiong Tao, Free fatty acid receptor GPR120 and pathogenesis of obesity and type 2 diabetes mellitus Progress in Molecular Biology and Translational Science. ,vol. 114, pp. 251- 276 ,(2013) , 10.1016/B978-0-12-386933-3.00007-8
Sarah-Jane Watson, Alastair J. H. Brown, Nicholas D. Holliday, Differential Signaling by Splice Variants of the Human Free Fatty Acid Receptor GPR120 Molecular Pharmacology. ,vol. 81, pp. 631- 642 ,(2012) , 10.1124/MOL.111.077388
Da Young Oh, Jung Ah Song, Jung Sun Moon, Mi Jin Moon, Jae Il Kim, Kyungjin Kim, Hyuk Bang Kwon, Jae Young Seong, Membrane-proximal region of the carboxyl terminus of the gonadotropin-releasing hormone receptor (GnRHR) confers differential signal transduction between mammalian and nonmammalian GnRHRs. Molecular Endocrinology. ,vol. 19, pp. 722- 731 ,(2005) , 10.1210/ME.2004-0220
Hong-Kui Wei, Yuanfei Zhou, Shuzhong Jiang, Ya-Xiong Tao, Haiqing Sun, Jian Peng, Siwen Jiang, Feeding a DHA-enriched diet increases skeletal muscle protein synthesis in growing pigs: association with increased skeletal muscle insulin action and local mRNA expression of insulin-like growth factor 1. British Journal of Nutrition. ,vol. 110, pp. 671- 680 ,(2013) , 10.1017/S0007114512005740
Atsuhiko Ichimura, Akira Hirasawa, Odile Poulain-Godefroy, Amélie Bonnefond, Takafumi Hara, Loïc Yengo, Ikuo Kimura, Audrey Leloire, Ning Liu, Keiko Iida, Hélène Choquet, Philippe Besnard, Cécile Lecoeur, Sidonie Vivequin, Kumiko Ayukawa, Masato Takeuchi, Kentaro Ozawa, Maithé Tauber, Claudio Maffeis, Anita Morandi, Raffaella Buzzetti, Paul Elliott, Anneli Pouta, Marjo-Riitta Jarvelin, Antje Körner, Wieland Kiess, Marie Pigeyre, Roberto Caiazzo, Wim Van Hul, Luc Van Gaal, Fritz Horber, Beverley Balkau, Claire Lévy-Marchal, Konstantinos Rouskas, Anastasia Kouvatsi, Johannes Hebebrand, Anke Hinney, Andre Scherag, François Pattou, David Meyre, Taka-aki Koshimizu, Isabelle Wolowczuk, Gozoh Tsujimoto, Philippe Froguel, Dysfunction of lipid sensor GPR120 leads to obesity in both mouse and human Nature. ,vol. 483, pp. 350- 354 ,(2012) , 10.1038/NATURE10798
Toshiki Tanaka, Susumu Katsuma, Tetsuya Adachi, Taka-aki Koshimizu, Akira Hirasawa, Gozoh Tsujimoto, Free fatty acids induce cholecystokinin secretion through GPR120. Naunyn-schmiedebergs Archives of Pharmacology. ,vol. 377, pp. 523- 527 ,(2008) , 10.1007/S00210-007-0200-8
He-Feng Luo, Hong-Kui Wei, Fei-Ruo Huang, Zheng Zhou, Si-Wen Jiang, Jian Peng, The Effect of Linseed on Intramuscular Fat Content and Adipogenesis Related Genes in Skeletal Muscle of Pigs Lipids. ,vol. 44, pp. 999- 1010 ,(2009) , 10.1007/S11745-009-3346-Y
Francisca Rodriguez-Pacheco, Sara Garcia-Serrano, Eva Garcia-Escobar, Carolina Gutierrez-Repiso, Juan Garcia-Arnes, Sergio Valdes, Montserrat Gonzalo, Federico Soriguer, Francisco J. Moreno-Ruiz, Alberto Rodriguez-Cañete, Jose L. Gallego-Perales, Abelardo Martinez-Ferriz, Gemma Rojo-Martínez, Eduardo Garcia-Fuentes, Effects of obesity/fatty acids on the expression of GPR120. Molecular Nutrition & Food Research. ,vol. 58, pp. 1852- 1860 ,(2014) , 10.1002/MNFR.201300666
Susumu Katsuma, Noriyuki Hatae, Takeaki Yano, Yoshinao Ruike, Mai Kimura, Akira Hirasawa, Gozoh Tsujimoto, Free fatty acids inhibit serum deprivation-induced apoptosis through GPR120 in a murine enteroendocrine cell line STC-1. Journal of Biological Chemistry. ,vol. 280, pp. 19507- 19515 ,(2005) , 10.1074/JBC.M412385200
Toshiki Tanaka, Takeaki Yano, Tetsuya Adachi, Taka-aki Koshimizu, Akira Hirasawa, Gozoh Tsujimoto, Cloning and characterization of the rat free fatty acid receptor GPR120: in vivo effect of the natural ligand on GLP-1 secretion and proliferation of pancreatic β cells Naunyn-Schmiedeberg's Archives of Pharmacology. ,vol. 377, pp. 515- 522 ,(2008) , 10.1007/S00210-007-0250-Y