AMINOACID RESIDUES INVOLVED IN POSITIVE MODULATION OF a1 GLYCINE RECEPTORS BY GINKGOLIC ACID

摘要: Previously, we have shown that ginkgolic acid has an ability to potentiate currents, mediated by αl subunits of glycine receptor. In order define the mechanism subunit specific action performed comparative analysis amino sequences at and α2 Amino acids contribute different on receptors formed these were determined. Using whole-cell configuration patch-clamp recording, demonstrated mutation three residues in for corresponding ones from makes sensitive potentiation acid. Currents, mutant receptors, increased 89±14% after application Similarly ai a decrease EC₅₀. under Thus, selectivity is strong connection with are α1 And