Drug Resistance Patterns and Genotyping of Acinetobacter baumannii Strains Isolated from Patients Admitted to Shahrekord Teaching Hospitals Using REP-PCR
作者： Atefe Sarafan Sadeghi , najmeh ansari , farzad khademi , Reza Mir Nejad , Behnam Zamanzad
关键词:
摘要: Background & objectives In recent years, Acinetobacter baumannii has been shown to be associated with several nosocomial infections, including pneumonia, bacteraemia, urinary tract wound infection and meningitis. This organism can survive in the hospital environment rapidly develops resistance many antibiotics. The molecular genotyping increase our knowledge about spread of A. strains from one another their drug resistance. Therefore, this study aimed determine prevalence antibiotic profile as well phylogenetic relationships Shahrekord teaching hospitals. Methods In study, antibacterial susceptibility patterns isolated different clinical specimens (urine, blood, sputum) amikacin, ampicillin/sulbactam, aztreonam, cefepime, ceftazidime, ciprofloxacin, gentamycin, imipenem, meropenem, norfloxacin, ofloxacin, piperacillin/tazobactam, tobramycin were tested using disk diffusion )Kirby-Bauer( method. Finally, was performed REP-PCR method. Results During 50 samples patients identified baumannii) 71%(, rates assessed. All resistant ceftazidime cefepime also a high rate ampicillin-sulbactam observed. On other hand, results demonstrated nine genotype groups among based on method. Conclusion Due strains, similarities between genotypes dispersion these parts hospitals, implementation control programs is necessary. Keywords: Acinetobacter , Antibiotic Resistance Genotyping
sci-hub.st 参考文章(7)
S. Misbah, S. AbuBakar, H. Hassan, Y.A. Hanifah, M.Y. Yusof, Antibiotic susceptibility and REP-PCR fingerprints of Acinetobacter spp. isolated from a hospital ten years apart Journal of Hospital Infection. ,vol. 58, pp. 254- 261 ,(2004) , 10.1016/J.JHIN.2004.07.007
Pavel Å vec, Marc Vancanneyt, Milan Seman, Cindy Snauwaert, Karen Lefebvre, Ivo SedláÄek, Jean Swings, Evaluation of (GTG)5-PCR for identification of Enterococcus spp. Fems Microbiology Letters. ,vol. 247, pp. 59- 63 ,(2005) , 10.1016/J.FEMSLE.2005.04.030
M. Ayan, R. Durmaz, E. Aktas, B. Durmaz, Bacteriological, clinical and epidemiological characteristics of hospital-acquired Acinetobacter baumannii infection in a teaching hospital. Journal of Hospital Infection. ,vol. 54, pp. 39- 45 ,(2003) , 10.1016/S0195-6701(03)00076-8
C. Lee Ventola, The Antibiotic Resistance Crisis: Part 1: Causes and Threats P & T : a peer-reviewed journal for formulary management. ,vol. 40, pp. 277- 283 ,(2015)
Petar Knezevic, Verica Aleksic, Natasa Simin, Emilija Svircev, Aleksandra Petrovic, Neda Mimica-Dukic, Antimicrobial activity of Eucalyptus camaldulensis essential oils and their interactions with conventional antimicrobial agents against multi-drug resistant Acinetobacter baumannii. Journal of Ethnopharmacology. ,vol. 178, pp. 125- 136 ,(2016) , 10.1016/J.JEP.2015.12.008
Maraisa G Delboni, Brenda PFA Gomes, Priscila A Francisco, Fabricio B Teixeira, David Drake, None, Diversity of Enterococcus faecalis Genotypes from Multiple Oral Sites Associated with Endodontic Failure Using Repetitive Sequence-based Polymerase Chain Reaction and Arbitrarily Primed Polymerase Chain Reaction. Journal of Endodontics. ,vol. 43, pp. 377- 382 ,(2017) , 10.1016/J.JOEN.2016.10.042
J Nowak, E Zander, D Stefanik, P G Higgins, I Roca, J Vila, M J McConnell, J M Cisneros, H Seifert, , High incidence of pandrug-resistant Acinetobacter baumannii isolates collected from patients with ventilator-associated pneumonia in Greece, Italy and Spain as part of the MagicBullet clinical trial Journal of Antimicrobial Chemotherapy. ,vol. 72, pp. 3277- 3282 ,(2017) , 10.1093/JAC/DKX322