Construction of a DNA-polymerase I overproducing plasmid and isolation of the enzyme.

摘要: The polA gene of Escherichia coli coding for DNA polymerase I was cloned under the control bacteriophage lambda promoter pL and N in a high copy number plasmid vector. chromosomally located cIts repressor kept synthesis product at 28 degrees C low level. Raising temperature to 43 resulted inactivation overproduction I, which could easily be purified homogeneity.